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LTP-RANKL |
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¥4000 ¥2500， 请电邮sales@lifetein.com.cn直接订购！ |
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小鼠 |
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每瓶25μg活性蛋白 |
| O35235 |
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大肠杆菌重组表达 |
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分子筛凝胶过滤分离 |
| 是 |
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根据其诱导小鼠单核细胞进行破骨细胞分化的生物学效果测定，EC50约为50ng/ml |
| 冷冻干燥蛋白粉末 |
| 使用包含0.1%以上的牛血清白蛋白的无菌PBS缓冲液进行复溶后使用 |
| 冻干粉可于零下20摄氏度或更低温度下保存12个月；复溶制剂可在4摄氏度下保存两周；避免反复冻融 |
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| A. The size-exclusion chromatography of recombinant mouse RANKL and the SDS-PAGE analysis. B. The bone marrow derived monocytes were cultured in the presence of M-CSF(25 ng/ml) and RANKL (50 ng/ml) for 6 days. The cells were then fixed with 4% PFA and stained by TRAP solution. The image showed the formation of big multiple nucleus TRAP positive osteoclasts formed after M-CSF and RANKL stimulation.
A. 重组小鼠RANKL蛋白的分子筛洗脱图谱与SDS-PAGE分析。B. 重组小鼠RANKL的活性分析。来自骨髓的单核细胞在添加本品(50 ng/ml)与M-CSF (25 ng/ml)的培养基中培养6天并随后进行PFA固定与TRAP染色成像。该图显示了使用本品刺激后巨型多细胞核形态的破骨细胞（TRAP+）的形成。
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| For research use only! |
