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首页 > 蛋白类产品 > 重组蛋白MCSF
重组蛋白,小鼠重组巨噬细胞集落刺激因子 / M-CSF / CSF-1
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LTP-MCSF |
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¥3500 ¥2000, 请电邮sales@lifetein.com.cn直接订购! |
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小鼠 |
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每瓶25μg活性蛋白 |
| P07141 |
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哺乳细胞分泌表达 |
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亲和层析联合分子筛凝胶过滤分离 |
| 是 |
| 根据其诱导小鼠单核细胞进行破骨细胞分化的生物学效果测定,ED50约为30ng/ml。 |
| 冷冻干燥蛋白粉末 |
| 使用包含0.1%以上的牛血清白蛋白的无菌PBS缓冲液进行复溶后使用 |
| 冻干粉可于零下20摄氏度或更低温度下保存12个月;复溶制剂可在4摄氏度下保存两周;避免反复冻融 |
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| A. The size-exclusion chromatography of recombinant mouse M-CSF/Fc chimera protein and the SDS-PAGE analysis. B. The bone marrow derived monocytes were cultured in the presence of M-CSF (30 ng/m) and RANKL (50 ng/ml, Cat. No. LTP-RANKL) for 6 days. The cells were then fixed with 4% PFA and stained by TRAP solution. The image showed the formation of big multiple nucleus TRAP positive osteoclasts formed after M-CSF and RANKL stimulation.
A. 重组小鼠M-CSF/Fc融合蛋白的分子筛洗脱图谱与SDS-PAGE分析。B. 重组小鼠M-CSF的活性分析。来自骨髓的单核细胞在添加本品(30 ng/ml)与RANKL(50 ng/ml,货号LTP-RANKL)的培养基中培养6天并随后进行PFA固定与TRAP染色成像。该图显示了使用本品刺激后巨型多细胞核形态的破骨细胞(TRAP+)的形成。
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| For research use only! |
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