Welcome to LifeTein Understanding Life, one protein at a time  
 

    服务和产品

 多肽合成服务

 GMP多肽合成

 多肽文库构建

 化学合成服务

 多肽合成试剂

 多肽库

 抗体定制服务

 抗体库

 
首页 > 抗体产品 > Anti V5 Monoclonal Antibody

Anti V5 Monoclonal Antibody

¥1550

download pdf data sheet  说明书下载

Calatog Number:LT9992
Lot Number:411005100208
Isotype:Mouse IgG1
Clonality:E10
Quantity:50 μg
Immunogen:GKPIPNPLLGLDST (V5) synthetic peptide conjugated to KLH
Specificity:Recognizes V5 (GKPIPNPLLGLDST) tagged fusion proteins
Purification:Protein A affinity chromatography from culture supernatant of hybridoma cells grown in a bioreactor
Applications:Dot, ELISA, IP, IS, WB
Sensitivity:WB (with ECL): 1-2ng of V5-tagged fusion proteins
Working Condition:WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
anti-V5 mAb dilution probed against HEK293 cells transfected with V5-tagged protein vector
Fig. 1:000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with V5-tagged protein vector; untransfected (1) and transfected (2)