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首页 > 抗体产品 > Anti GFP Monoclonal Antibody
Anti GFP Monoclonal Antibody
¥1350 | |
Calatog Number: | LT9994 |
Lot Number: | 411005100210 |
Isotype: | Mouse IgG1 |
Clonality: | GF28R |
Quantity: | 50 μg |
Immunogen: | GFP from the jellyfish Aequorea Victoria N-terminal peptide-KLH conjugates |
Specificity: | Recognizes native and denatured forms of GFP and its variants such as: EGFP, YFP, EYFP, and CFP |
Purification: | Protein A affinity chromatography from mouse ascites fluid |
Applications: | Dot, ELISA, IP, IS, WB |
Sensitivity: | WB (with ECL): 1-10ng of purified GFP or GFP fusion proteins |
Working Condition: | WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature) IS: 1:500-2000 dilution For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test |
Storage: | Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks |
Images: | Fig. 1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with GFP-tagged protein vector: untransfected control (1), 1µg (2) and 10µg (3) of cell lysates used |
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