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首页 > 抗体产品 > Anti GAPDH Monoclonal Antibody

Anti GAPDH Monoclonal Antibody

¥1500
Calatog Number:LT9995
Lot Number:411005100211
Isotype:Mouse IgG1
Clonality:GA1R
Quantity:50 μg
Immunogen:Recombinant GAPDH
Specificity:Recognizes native and denatured forms of GAPDH (~37kDa)
Cross Reactivity:GAPDH from BL-21 bacteria, Sf9 insect, Saccharomyces cerevisiae (yeast), human, mouse, rat, rabbit, chicken, and hamster. GAPDH from other species may also be detectable
Purification:Protein A affinity chromatography from mouse ascites fluid
Applications:Dot, ELISA, IS, WB
Working Condition:WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
anti-gadph mAb dilution used in WB of lane tissue lysates from human etc.anti-gadph mAb dilution used in WB of lane tissue lysates from human etc.
Fig. LEFT: 1:2000 (0.5µg/mL) Ab dilution used in WB of 5µg/lane tissue lysates from human (1), mouse (2), rat (3), rabbit (4), chicken (5), and hamster (6). RIGHT: WB from BL-21 bacteria (1), Sf9 insect (2), and Saccharomyces cerevisiae (3)