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首页 > 抗体产品 > Anti DYKDDDDK(FLAG) Monoclonal Antibody

Anti Anti-DYKDDDDK(FLAG) Monoclonal Antibody

¥1250
Calatog Number:LT0420
Lot Number:411005100203
Isotype:Mouse IgG2b
Clonality:FG4R
Quantity:50 μg
Immunogen:DYKDDDDK (FLAG) synthetic peptide conjugated to KLH
Specificity:Recognizes the N-terminal, C-terminal or internal DYKDDDDK-tagged fusion proteins
Purification:Protein A affinity chromatography from mouse ascites fluid
Applications:Dot, ELISA, IS, IP, WB
Sensitivity:WB (with ECL): 1-5ng of DYKDDDDK-tagged fusion proteins
Working Condition:WB (with ECL): 1:1000-3000 dilution (incubate for 1 hour at room temperature)
IS: 1:500-2000 dilution
For best results with other assays (e.g.: Dot, ELISA, IP, etc), please determine optimal working dilution by titration test
Storage:Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
anti-FLAG mAb dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector
Fig. 1:1000 (1µg/mL) Ab dilution probed against HEK293 cells transfected with DYKDDDDK-tagged protein vector; untransfected (1) and transfected (2)