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首页 > 抗体产品 > Anti ERK1 (E32) Monoclonal Antibody

Anti ERK1 (E32) Monoclonal Antibody

¥1600
Calatog Number:LT9997
Lot Number:411005100213
Isotype:Mouse IgG1
Clonality:E32
Quantity:50 μg
Immunogen:Recombinant full length ERK1 protein
Specificity:Recognizes only the ERK1 protein (e.g.: from human and mouse cells)
Cross Reactivity:WB: ERK1 from human and mouse cells. IP: ERK1 from human cells. ERK1 from other species may also be detectable
Purification:Protein A affinity chromatography from mouse ascites fluid
Applications:IP, WB
Working Condition:WB (with ECL): 1:1000-10,000 dilution (incubate for one hour at room temperature)
For best results with other assays (e.g.: IP, etc), please determine optimal working dilution by titration test
Storage:Store at -20°C for up to 2 years. Centrifuge after first thaw to maximize product recovery. Aliquot to avoid repeated freeze-thaw cycles. Store aliquots at 4°C for 1-2 weeks
Images:
anti-ERK1 (E32) mAb dilution used in WB of HEK293 and HELA cell lysateanti-ERK1 (E32) mAb dilution used in WB of HEK293 and HELA cell lysate
Fig. LEFT: 1:1,000 (1µg/mL) Ab dilution used in WB of HEK293 cell lysate; 5µg (1), 10µg (2), and 30µg (3) of cell lysate used; RIGHT: IP of anti-ERK1 (1µL) using HeLa cell lysate; 10µg (1), 25µg (2), and 50µg (3) of cell lysate used