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It is easy. Just email us a protein sequence and we will send you minigrams of proteins. |
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It is flexible. If the 6His tag does not give us efficient expression, we will change the 6His tag to GST for free. |
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It is economical. We offer package price from gene synthesis, cloning, protein expression to crystallization or antibody production. |
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It is productive. Optimized refolding methodology is available to solve the protein solubility issues. Enhanced expression vectors are used to increase the protein expression scale and bioactivity. |
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It is large. Need more proteins? We are able to scale up the protein expression to hundreds of litres or gram levels. |
Features
- Bacterial Expression System: Different optimized conditions are used for clones in order to find the optimum soluble expression condition with our system. The pET expression system provides high level of protein production.
- E. coli system for soluble protein with high stability
- Classic ploy-Histidine tag for most protein of interest
- High affinity FLAG or GST tag for protein with very low expression level
- Baculovirus Insect Cell Expression System:
- Insect cell system to meet the demand for economic and large amount of eukaryotic proteins in their native conformation
- Different purification tags on customer’s request
- Cell culture in Sf9 or High Five suspension culture cell lines for optimum expression
Frequently Asked Questions: Protein expression, purification and crystallization
1. How long does a baculovirus expression project usually take?
Starting with the baculovirus expression plasmid, our service can be completed in 8-9 weeks.
2. What advantages does a baculovirus expression system provide?
Baculovirus expression systems can be used when a target protein with posttranslational modifications is needed for functional and structural studies. While peptide synthesis is usually for difficult proteins that are not feasible within any protein expression system. In addition, the eukaryotic Baculovirus/Insect system is completely safe.
3. What if my gene does not express well?
We have different expression strains and leader sequences for optimizing expression. Options of refolding methods and tags are used for the best conditions.
LifeTein Refolding Technology
Recombinant proteins accumulated intracellularly form inclusion bodies, or insoluble aggregates of misfolded protein lacking biological activity. Proteins have to fold in a correct conformation. In this way, specific sites of the right size, shape, and polarity are created for their ligands or receptors. Conventional methods for refolding of insoluble recombinant proteins include slow dialysis, ion exchange, gel filtration, or dilution into a buffer of near neutral pH.
LifeTein's refolding technology is based on a simple and historically successful protocol. This method reduces protein waste and minimizes unnecessary analysis. The simplified protocol provides reliable, high-yield and high concentration of refolded proteins.